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A direct method for quantification of non-transferrin-bound iron

✍ Scribed by Surinder Singh; Robert C. Hider; John B. Porter

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
477 KB
Volume
186
Category
Article
ISSN
0003-2697

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✦ Synopsis

direct method for quantification of non-transferrin-bound iron has been developed. This assay relies on the use of a large excess of a low affinity ligand (nitrilotriacetic acid, NTA) which removes and complexes all low molecular weight iron and iron nonspecifically bound to serum proteins. Iron bound to transferrin, ferritin, desferrioxamine, and its metabolites is unaffected. The Fe-NTA complex present in the serum ultrafiltrate is then quantified using an automated HPLC procedure where on-column derivatization with a high affinity iron chelator (3-hydroxy-1-propyl-a-methylpyridin-4-one) takes place. The iron complexes of desferrioxamine and its metabolites are unaffected by the above-derivatization procedure. With minor modifications, this method is equally applicable for the quantification of low molecular weight iron in other biological fluids.

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