𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Density regulation of amino acid transport in cultured, androgen-responsive tumour cells

✍ Scribed by Jeffery H. Robinson

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
541 KB
Volume
89
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

In androgen‐responsive cells, activity of a sodium‐dependent transport system for neutral amino acids is shown to be density‐dependent, whereas activity of a sodium‐independent system is not. Transport of the nonmetabolizable amino acid, α‐aminoisobutyric acid (α‐aib) was largely sodium‐dependent, whereas transport of cycloleucine (cyleu) was by both sodium‐dependent and sodium‐independent systems. The K~m~ for cyleu transport changed with density because only the sodium‐dependent portion of this transport was density‐dependent. By contrast, the V~max~ for α‐aib transport changed with density, indicating that either the amount of functional membrane carrier or the activity of the sodium pump was affected.

📜 SIMILAR VOLUMES

Serum-mediated regulation of amino acid
Serum-mediated regulation of amino acid transport in cultured chick embryo fibroblasts
✍ Mariarosaria Tramacere; Angelo F. Borghetti; Guido G. Guidotti 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 906 KB

## Abstract When chicken serum was added to serum‐deprived quiescent cultures of chick embryo fibroblasts the activity of amino acid transport by means of the A system, as measured by α‐aminoisobutyric acid and L‐proline uptake after discrimination of the contribution of interacting systems, increa

Hormonal regulation of amino acid transp
Hormonal regulation of amino acid transport and gluconeogenesis in primary cultures of adult rat liver parenchymal cells
✍ Rolf F. Kletzien; Michael W. Pariza; Joyce E. Becker; Van R. Potter 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 496 KB

Amino acid transport was studied in primary cultures of parenchymal cells isolated from adult rat liver by a collagenase perfusion technique and maintained as a monolayer in a serum-free culture medium. These cells carried out gluconeogenesis from three carbon precursors (alanine, pyruvate, and lact

Hormonal regulation of amino acid transp
Hormonal regulation of amino acid transport and cAMP production in monolayer cultures of rat hepatocytes
✍ Darshan S. Kelley; James D. Shull; Van R. Potter 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 847 KB

## Abstract The effects of insulin, glucagon or Dexamethasone (DEX) and of glucagon with insulin or DEX were examined on the uptake of 2‐amino [1‐^14^C]isobutyric acid (AIB) and N‐Methyl‐2‐amino [1‐^14^C]isobutyric acid (NMe AIB) in monolayer cultures of rat hepatocytes. Insulin and glucagon stimul

Adaptive regulation of neutral amino aci
Adaptive regulation of neutral amino acid transport system A in rat H4 hepatoma cells
✍ Michael S. Kilberg; He-Ping Han; Ellen F. Barber; Thomas C. Chiles 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 889 KB

Substrate regulation of System A transport activity in rat H4 hepatoma cells is described. The uptake of several amino acids was tested in t h e presence of system-specific inhibitors. System A activity was increased in a RNA-and protein synthesis-dependent manner by amino acid deprivation of the ce

Growth regulation and amino acid transpo
Growth regulation and amino acid transport in epithelial cells: Influence of culture conditions and transformation on A, ASC, and l transport activities
✍ Paula Boerner; Milton H. Saier Jr. 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 967 KB

## Abstract Amino acid transport in Madin‐Darby canine kidney (MDCK) cells, grown in a defined medium, was investigated as a function of cell density, exposure to specific growth factors, and transformation. MDCK cells were found to transport neutral amino acids by systems similar to the A, ASC, L,