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Density gradient centrifugation of hemopoietic colony-forming cells

โœ Scribed by R. W. A. Turner; L. Siminovitch; E. A. McCulloch; J. E. Till

Publisher
John Wiley and Sons
Year
1967
Tongue
English
Weight
633 KB
Volume
69
Category
Article
ISSN
0021-9541

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โœฆ Synopsis

Buoyant density distributions of hemopoietic colony-forming units (CFU) from normal mouse marrow were determined by equilibrium density gradient centrifugation in bovine serum albumin (BSA) gradients. The distributions were compared with those obtained for the total population of nucleated cells from normal mouse marrow. The buoyant density distribution for CFU was found to differ from the density distribution for the total nucleated cell population, and the portion of the total cell population with densities much less than the mean value was found to contain up to a 30-fold greater proportion of CFU than an uncentrifuged control. These results provide a preliminary approach to the purification and characterization of normal hemopoietic colony-forming stem cells.

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