Density distribution and cation composition of red blood cells in newborn puppies

✍ Scribed by Ping Lee; P. R. Miles

• Publisher: John Wiley and Sons
• Year: 1972
• Tongue: English
• Weight: 802 KB
• Volume: 79
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040790308

✦ Synopsis

The density distribution and cation composition of red blood cells from newborn puppies have been studied. The density distribution of red cells from a newborn puppy in a bovine serum albumin density gradient resembles a normal distribution with a peak density at a region less than that found for adult dog red cells. In two weeks the whole distribution shifts toward a more dense region, and a second cell peak appears so that the distribution becomes bimodal. This second cell peak is smaller than the original peak, and it appears at a region of lower density. In nine weeks the distribution becomes a normal one again, but the peak density corresponds to the peak density of the second cell peak which first appeared at two weeks. Evidence has been obtained to show that fetal red cells are located in the more dense cell peak and neonatal cells are in the less dense second peak. These results were obtained by labeling fetal cells with Cr5I and neonatal cells with Fe59. The analysis of the