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35Cl NMR Study of Cl− Distribution and Transport in Human Red Blood Cell Suspensions

✍ Scribed by Wanrong Lin; Duarte Mota de Freitas

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 604 KB
Volume: 34
Category: Article
ISSN: 0749-1581
DOI: 10.1002/(sici)1097-458x(199610)34:10<768::aid-omr964>3.0.co;2-7

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✦ Synopsis

The chloride (Cl-) distribution in human red blood cell (RBC) suspensions was studied using cobalt(I1) glycine, [ Co(Gly), 1 -, as a shift reagent (SR). When [ Co(Gly), 1 -was added to suspensions of human RBCs, it shifted the ,'C1 NMR signal of the extracellular C1-. Intracellular and extracellular 35C1-signals were clearly discriminated. Erythrocytes were impermeable to [ Co(Gly),l-but were slowly permeable to the SR Coz+ and to the relaxation agent Mn2+. The exchange of intracellular C1-with extracellular hypophosphite in human RBCs was monitored by ,'CI NMR. The C1-efflux was found to follow first-order kinetics with an efflux rate constant of 0.0024 min-'.

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Diffusion in red blood cell suspensions: Separation of the intracellular and extracellular NMR sodium signal

✍ J. W. C. van der Veen; P. van Gelderen; J. H. N. Creyghton; W. M. M. J. Bovée 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 375 KB

## Abstract It is shown that the signal of intracellular and extracellular sodium of red blood cells can be separated by a difference in diffusion. Comparison with proton diffusion experiments conducted in parallel showed that this difference was caused by restriction to the cell volume. The measur