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Denaturation of horseradish peroxidase with urea and guanidine hydrochloride

โœ Scribed by A.A. Moosavi-Movahedi; K. Nazari

Book ID
116091639
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
424 KB
Volume
17
Category
Article
ISSN
0141-8130

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Eleven mutant forms of staphylococcal nuclease with one or more defined amino acid substitutions have been analyzed by solvent denaturation by using intrinsic fluorescence to follow the denaturation reaction. On the basis of patterns observed in the value of m--the rate of change of log Kapp (the ap