Demonstration of the lack of acrosin in mouse spermatozoa by a radioassay
β Scribed by Robert P. Erickson; Susan R. Martin
- Book ID
- 115842976
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 309 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0010-7824
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## Abstract An immunoperoxidase staining procedure that readily demonstrated acrosin in the rostral portion of the acrosome failed to detect acrosin in the equatorial segment of spermatozoa representing the mammalian orders artiodactyla (bull and boar), lagomorpha (rabbit) and primate (human).
A nuclear DNA-polymerase activity has been detected in situ in mouse spermatozoa with a cytochemical method. The acid-insoluble radioactive product obtained after incubation in a mixture containing all four deoxyribonucleoside 5'-triphosphates, Mg++,KCl and dithiothreitol (DTT) was completely remove