Demonstration of rheumatoid factor in tissue by mixed agglutination with tissue sections

✍ Scribed by E. J. Milde; O. TÖNder

• Publisher: John Wiley and Sons
• Year: 1968
• Tongue: English
• Weight: 701 KB
• Volume: 11
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780110403

✦ Synopsis

MILDE AND 0. TONDER The mixed agglutination procedure was applied to sections of synovial tissue from patients with rheumatoid arthritis. The tissues were examined with three test systems: 1) Sheep erythrocytes sensitized by rabbit antiserum, 2) human 0 Rh-positive (CD) erythrocytes sensitized by anti-Rh serum "Ripley," and 3) rabbit erythrocytes sensitized with mercaptoethanol-treated pooled human serum. Adsorption of the sensitized cells to the tissue sections was SING fluorescent antibody technique, U rheumatoid factor (RF) has been demonstrated in the synovial membrane, lymph nodes and subcutaneous nodules of patients suffering from rheumatoid arthritis.1,2.3-4 RF has also been demonstrated on the surface of leukocytes from peripheral blood5 and in leukocytes from synovial f l ~i d . ~, ~~* , @

Recent observations indicate that uptake of complexes containing RF by peripheral blood leukocytes in uitro results in disruption of lysosomes and release of enzymes.1° Accordingly RF may play a part in the inflammatory reactions occurring in the tissue of patients with rheumatoid a r t h r i t i ~. ~J ~J ~

In spite of accumulated knowledge concerning RF in serum, information about RF in the tissue is still inadequate. We