Demonstration of monoclonal origin of human parotid gland pleomorphic adenoma

✍ Scribed by Shinzaburo Noguchi; Tomohiko Aihara; Kunitoshi Yoshino; Kazuyoshi Motomura; Hideo Inaji; Shingi Imaoka; Hiroki Koyama

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 452 KB
• Volume: 77
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/(sici)1097-0142(19960201)77:3<431::aid-cncr2>3.0.co;2-n

✦ Synopsis

BACKGROUND.

Parotid gland pleomorphic adenoma is histologically comprised of epithelial and mesenchymal elements. It remains to be established whether this neoplasm arises from epithelial and mesenchymal elements, or solely from the epithelial element.

METHODS.

In an attempt to resolve this issue, we have conducted clonal analysis on five pleomorphic adenomas. The method for clonal analysis was based on the trinucleotide repeat polymorphism of the x-chromosome-linked androgen receptor gene and on random inactivation of this gene by methylation. The epithelial and mesenchymal elements were obtained separately from the paraffin sections of the pleomorphic adenomas using a microdissection technique and then subjected to clonal analysis.

RESULTS.

Clonal analysis revealed that both epithelial and mesenchymal elements were monoclonal. In addition, the same allele of the androgen receptor gene was inactivated in both elements in every case.