Demonstration of hepatitis B virus DNA using polymerase chain reaction in serum and liver after loss of HBs Ag induced by antiviral therapy
β Scribed by Marcellin, P.; Martinot-Peignoux, M.; Loriot, M.A.; Giostra, E.; Bover, N.; Benhamou, J.P.
- Book ID
- 122668149
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 51 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0168-8278
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Sera from 98 patients with various stages of chronic hepatitis B virus infection were studied to determine the clinical significance of hepatitis B virus DNA in serum detected by the polymerase chain reaction. Patients were divided into three groups according to their HBsAg and HBeAg status. Group I
We developed a polymerase chain reaction assay for the direct detection of hepatitis B virus in paraffin-embedded liver tissue and applied this assay to determine whether hepatitis B virus DNA exists in livers with chronic hepatitis non-A, non-B. Fifty five liver biopsy samples were studied: 11 from