Use of the polymerase chain reaction to demonstrate hepatitis B virus DNA in serum of children with chronic hepatitis B
✍ Scribed by Stefan Wirth; Ulrike Möllers; Klaus-Michael Keller; Andreas Winterpacht
- Book ID
- 117162584
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 258 KB
- Volume
- 120
- Category
- Article
- ISSN
- 1097-6833
No coin nor oath required. For personal study only.
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The polymerase chain reaction (PCR) technique has been utilized for the detection of hepatitis B virus (HBV) DNA, and several factors related to the selection of primer pairs for the PCR amplification have been demonstrated. The sensitivity of the PCR assay was compared with that of slot-blot hybrid
We developed a polymerase chain reaction assay for the direct detection of hepatitis B virus in paraffin-embedded liver tissue and applied this assay to determine whether hepatitis B virus DNA exists in livers with chronic hepatitis non-A, non-B. Fifty five liver biopsy samples were studied: 11 from
## Abstract The patterns of hepatitis B virus DNA (HBV‐DNA) expression were studied in 2 sequential liver biopsies from 26 children (18 treated with interferon and 8 controls) with chronic hepatitis B. In the basal biopsy replicative forms of HBV‐DNA were detected in all of the samples and integrat