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Demonstration and solubilization of an inhibitor of hepatic cholesterol biosynthesis from rat liver microsomes

✍ Scribed by Juli R. Krzemien; Guy T. Haven


Book ID
115736903
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
746 KB
Volume
369
Category
Article
ISSN
0005-2760

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Solubilization of 3-hydroxy-3-methylglut
✍ Zafarul H. Beg; John A. Stonik; H.Bryan Brewer Jr. πŸ“‚ Article πŸ“… 1978 πŸ› Elsevier Science 🌐 English βš– 312 KB

A simple, efficient, freeze-thaw procedure for the solubilization of liver 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase has been developed. Microsomes of chicken or rat liver were prepared by homogenization in buffer containing 100 mM sucrose, 50 mM KCI, 40 mM KH,PO,, 30 mM EDTA. and 2