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Deletion mapping of 14q32 in human neuroblastoma defines an 1,100-kb region of common allelic loss

✍ Scribed by Hoshi, Masato ;Shiwaku, Hiromi O. ;Hayashi, Yutaka ;Kaneko, Yasuhiko ;Horii, Akira

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 152 KB
Volume: 35
Category: Article
ISSN: 0098-1532
DOI: 10.1002/1096-911x(20001201)35:6<522::aid-mpo4>3.0.co;2-6

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✦ Synopsis

Background:

We analyzed loss of heterozygosity (loh) in 54 primary neuroblastomas (nbs) using 12 microsatellite markers on 14q32, and found that 31% (17/54) nbs showed loh.

Procedure:

The smallest region of overlap (sro) was identified between d14s62 and d14s987.

Results:

There was no statistical correlation between loh and any clinicopathologic features, including age, stage, amplification of mycn, and ploidy. a sequence-ready bacterial artificial chromosome (bac) contig was constructed, and the minimum tiling path of six bacs covered the sro; the physical length of this region was no larger than 1,000 kb.

Conclusions:

Our findings support the existence of a putative tumor-related gene on 14q32 for the tumorigenesis of nb.

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