Degradation pathways of salmon calcitonin in aqueous solution
✍ Scribed by Vincent Windisch; Frank Deluccia; Laurent Duhau; Frédéric Herman; James J. Mencel; Sheng-Yuh Tang; Marc Vuilhorgne
- Book ID
- 109388635
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 152 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0022-3549
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Degradation impurities have been investigated, by means of high-performance liquid chromatography (HPLC)/ mass spectrometry, in commercial ampoules of salmon calcitonin, aqueous solution, stored for up to 2 years. Oxidation derivatives of the cysteine residues in positions 1 and 7, with cleavage of
Salmon calcitonin, a 32-residue peptide with a 1-7 disulfide bridge, was synthesized by standard solid-phase techniques, and studied by CD and two-dimensional NMR experiments. The peptide was dissolved in pure trifluoroethanol ( T F E ) and in aqueous solutions containing various amounts of TFE. CD