✦ LIBER ✦

High-performance Liquid Chromatography/Tandem Mass Spectrometry Identification of Salmon Calcitonin Degradation Products in Aqueous Solution Preparations

✍ Scribed by Luigi Silvestro; Simona Rizea Savu

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 562 KB
Volume: 10
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19960131)10:2<151::aid-rcm434>3.0.co;2-c

No coin nor oath required. For personal study only.

✦ Synopsis

Degradation impurities have been investigated, by means of high-performance liquid chromatography (HPLC)/ mass spectrometry, in commercial ampoules of salmon calcitonin, aqueous solution, stored for up to 2 years.

Oxidation derivatives of the cysteine residues in positions 1 and 7, with cleavage of the disulfide bridge, were observed in ail samples. Using our reversed-phase separation, these oxidation derivatives coeluted and presented an HPLC behavior identical to the derivative of salmon calcitonin with the disulfide bridge reduced. In a previous study this last compound was tentatively identified, without confirmation by mass spectrometry, as a major degradation product of salmon calcitonin in aqueous solution.

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