Degradation of busulfan in aqueous solution

✍ Scribed by Moustapha Hassan; Hans Ehrsson

• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 437 KB
• Volume: 4
• Category: Article
• ISSN: 0731-7085
• DOI: 10.1016/0731-7085(86)80027-9

✦ Synopsis

The influence of pH, phosphate buffer components and temperature on the degradation rate of busulfan was studied. The analysis was performed using gas chromatography with electron capture detection and reversed-phase liquid chromatography with radioactivity monitoring. The degradation rate of busulfan showed no pH dependence in the range pH 1.5-11 and increased at higher pH values. The degradation rate constant was 0.034 'c-0.001 hh' (S.E.M.) for the degradation of busulfan in pure water and 0.45 f 0.01 h-'M-' (S.E.M.) for the reaction of busulfan with the hydroxide ion at 37°C. The reactivity of HP04-2 was six tjmes higher than the reactivity of H2PO4-i towards busulfan. The hydrolysis products were identified as tetrahydrofuran and methanesulphonic acid by nuclear magnetic resonance spectroscopy.