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Defined medium for primary culture de novo of adult rat alveolar epithelial cells

✍ Scribed by Zea Borok; Spencer I. Danto; Stephanie M. Zabski; Edward D. Crandall

Book ID
112801870
Publisher
Society for In Vitro Biology
Year
1994
Tongue
English
Weight
742 KB
Volume
30
Category
Article
ISSN
1475-2689

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## Abstract Primary cultures of baby mouse kidney epithelial cells can grow without fibroblast overgrowth in a hormone‐supplemented serum‐free medium (Medium K‐1) designed for an established kidney epithelial cell line, MDCK. The five supplements in Medium K‐1 are insulin, transferrin, PGE~1~, T~3~