De Novo expression of intercellular adhesion molecule 1(ICAM-1, CD54) in pancreas cancer

✍ Scribed by Wilhelm Schwaeble; Mariola Kerlin; Karl-Hermann Meyerzum Büschenfelde; Wolfgang Dippold

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: French
• Weight: 792 KB
• Volume: 53
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910530226

✦ Synopsis

Abstract

We examined the expression of intercellular—adhesion molecule‐I (ICAM‐I, CD54) in 6 surgically removed pancreatic tumors and 8 pancreatic tumor cell lines. Immunohistochemistry revealed a varying percentage of ICAM‐I‐positive pancreas tumor cells, while normal pancreatic tissue (except for slight reactivity of endothelial cells) was not stained. The presence of the ICAM‐I molecule on the cell surface and the expression of ICAM‐I mRNA were investigated for 8 different pancreatic tumor cell lines. Three of these (Capan‐I, Capan‐2, QGP‐I) expressed ICAM‐I constitutively. In 4 of the ICAM‐I‐negative pancreas cancer cell lines, it was possible to induce a remarkable expression of ICAM‐I by incubating the cells in the presence of inflammatory cytokines, whereas one cell line, 818‐4, remained ICAM‐I‐negative. The responsiveness to either IFN‐γ, TNF‐α, or IL‐Iβ treatment was shown to vary from cell line to cell line, indicating complex mechanisms that regulate the expression of ICAM‐I at both, the transcriptional and the post‐transcriptional level. Interestingly, ICAM‐I is shed by pancreatic tumor cells, since soluble SICAM‐I was detected in the cell‐culture supernatants. In comparison with normal sera, the mean level of sICAM‐I in sera of patients with pancreas carcinoma is elevated 2‐fold.