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Data-driven normalization strategies for high-throughput quantitative RT-PCR

✍ Scribed by Jessica C Mar; Yasumasa Kimura; Kate Schroder; Katharine M Irvine; Yoshihide Hayashizaki; Harukazu Suzuki; David Hume; John Quackenbush

Book ID
114998626
Publisher
BioMed Central
Year
2009
Tongue
English
Weight
636 KB
Volume
10
Category
Article
ISSN
1471-2105

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## Abstract Careful validation of reference genes used for the normalization of real‐time RT‐PCR data is required to obtain accurate results regarding gene expression. We evaluated the stability of seven commonly used reference genes in the cerebral cortex and hippocampus of rats 3 days following t