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Darstellung von oligothymidinphosphaten durch chemische partialhydrolyse von desoxyribonucleinsäure

✍ Scribed by Schott, Herbert

Publisher
Wiley (John Wiley & Sons)
Year
1981
Weight
747 KB
Volume
182
Category
Article
ISSN
0025-116X

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✦ Synopsis

Abstract

The method of preparation of oligo(thymidine phosphate)s by chemical partial hydrolysis of herring sperm DNA is described. The DNA is degraded in three steps to a mixture of oligomeric thymidine phosphates. Homologeous thymidine phosphates of the series p(dT)~1–6~p; p(dT)~1–7~, (dT)~1–7~p; (dT)~1–7~ are isolated chromatographically from the partial hydrolysate in pure form on a preparative scale. The advantages and disadvantages of this new and simple method in comparison to complicated synthetic procedures are discussed.

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