The major DNA photoproduct formed upon uv irradiation of Bacillus subtilis spores is the thymine dimer 5-thyminyl-5,6-dihydrothymine, informally referred to as spore photoproduct (SP). A rapid separation technique for detecting and quantitating SP by HPLC was developed to replace traditional paper c
Dark repair of DNA containing ?spore photoproduct? in Bacillus subtilis
โ Scribed by Munakata, Nobuo ;Rupert, Claud S.
- Publisher
- Springer
- Year
- 1974
- Tongue
- English
- Weight
- 777 KB
- Volume
- 130
- Category
- Article
- ISSN
- 0026-8925
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A DNA protein complex has been isolated from vegetative cells and spores of Bacillus subtilis. Properties of the DNA protein complex prepared from vegetative cells were studied and SDS gel electrophoresis was employed to compare the different DNase-untreated and -treated DNA protein complexes. It is
Spores of Bacillus subtilis are approximately ten times less likely to survive UV light irradiation in a vacuum than under atmospheric conditions. Photoproduct formation was studied in spores irradiated under ultrahigh vacuum (UHV) conditions and in spores irradiated at atmospheric pressure. In addi