Bacillus subtilis forms both vegetative cells and spores. The Β―uidity of the membranes in these forms was measured by using Β―uorescent anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH). The spores were more rigid than the vegetative cells, suggesting that the structure of the spores and vegetative c
β¦ LIBER β¦
Differences in pattern of a DNA protein complex isolated from vegetative cells and spores of Bacillus subtilis
β Scribed by Gruissem, Wilhelm
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 507 KB
- Volume
- 159
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
A DNA protein complex has been isolated from vegetative cells and spores of Bacillus subtilis. Properties of the DNA protein complex prepared from vegetative cells were studied and SDS gel electrophoresis was employed to compare the different DNase-untreated and -treated DNA protein complexes. It is concluded that proteins are associated with the DNA and differences in protein pattern in polyacrylamide gels indicates the involvement of DNA-binding proteins in the regulation of spore formation.
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