Damaged Products of Thymine in Hydroxyl Radical Solution under UV Irradiation

✍ Scribed by Jongki Hong; Do-Gyun Kim; Jong Shin Yoo; Chaejoon Cheong

• Book ID: 102614703
• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 71 KB
• Volume: 63
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1006/mchj.1999.1772

✦ Synopsis

Modification of DNA bases in a hydrogen peroxide solution by UV irradiation of thymine was investigated. Twelve products resulting from interaction of radicals with thymine were identified by using gas chromatography/mass spectrometry (GC/MS) after trimethylsilyl derivatization. Some products were identified by comparing their retention times and mass spectra with those of synthetic compounds that are formed by treatment of thymine with OsO 4 . Major products were quantified by the GC/MS-selected ion monitoring mode. We also examined the effect of length of UV irradiation and amount of hydrogen peroxide on the quantitation of a number of DNA base-damaged products. The results indicate that the radical environment provided by the presence of hydrogen peroxide has a substantial effect on the types of products as well as their quantities. Moreover, the presence of hydrogen peroxide dramatically induced high yields of oxidative products.