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Cytotoxicity of novel unsymmetrically substituted inhibitors of polyamine biosynthesis in human cancer cells

✍ Scribed by Lynsey M. Nairn; Gayle S. Lindsay; P.M. Woster; Heather M. Wallace

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 133 KB
Volume: 182
Category: Article
ISSN: 0021-9541
DOI: 10.1002/(sici)1097-4652(200002)182:2<209::aid-jcp9>3.0.co;2-8

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✦ Synopsis

The cytotoxicity of two novel polyamine analogues was compared with that of a known cytotoxic drug, etoposide, in a human promyelogenous leukemic cell line. CHEN-spm showed significant acute cytotoxicity in these cells and was comparable to etoposide in terms of IC 50 value. The cell death observed from both CHEN-spm and etoposide was typically apoptotic with increased DNA fragmentation, altered cell morphology, and cell cycle distribution. CPEN-spm, on the other hand, exhibited no toxic effects over the short-term (24 h) exposure period. Intracellular polyamine content decreased in the presence of all inhibitors but only CPEN-spm produced significant induction of spermidine/spermine N 1acetyltransferase in 24 h. Thus, increased polyamine catabolism appears not to be essential for the initiation of apoptotic cell death in these human leukemic cells.

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Erratum: Nairn LM, Lindsay GS, Woster PM

Erratum: Nairn LM, Lindsay GS, Woster PM, and Wallace HM (2000): Cytotoxicity of novel unsymmetrically substituted inhibitors of polyamine biosynthesis in human cancer cells. J. Cell Physiol 182:209-213.

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