𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Cytotoxicity evaluation of an antibacterial dentin adhesive system on established cell lines

✍ Scribed by Elizabeth A. Koulaouzidou; Maria Helvatjoglu-Antoniades; George Palaghias; Artemis Karanika-Kouma; Dimitrios Antoniades

Book ID
102297300
Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
269 KB
Volume
84B
Category
Article
ISSN
1552-4973

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Clearfil Protect Bond is a new dental bonding agent recently introduced into clinical practice. It contains an antibacterial monomer that contributes to its antibacterial profile. The aim of the present study was to evaluate cytotoxic effect of Clearfil Protect Bond against three established fibroblastic cell lines, in comparison with four commonly used adhesive materials (Adper Scotchbond 1, Excite, Tyrian SPE, and One Step plus). The experiments were performed using RPC‐C2A, BHK21/C13, and MRC5 cell lines. Test specimens, either cured or uncured, were placed in a culture medium and the extraction media were used as experimental material. The effect of the bonding materials was assessed by a modified sulforhodamine‐B assay after 24 and 48 h of exposure. All tested agents exhibited an antiproliferative effect on cells, the effect on RPC‐C2A being the most marked. Extraction media from the uncured materials were without exception highly cytotoxic. In the experiments performed using extraction medium from cured material, Clearfil Protect Bond appeared to be the least toxic material, followed by Tyrian SPE and One Step plus. Adper Scotchbond 1 and Excite exhibited the strongest cytotoxic effect. The cell survival percentage ranged between 66 and 97% for Clearfil Protect bond, 15 and 82% for Tyrian SPE, 28 and 58% for One Step plus, 2 and 28% for Excite, and 1 and 6% for Adper Scotchbond 1. Taking into consideration the limitations of an in vitro study, our results indicate that the new antibacterial dental adhesive system is suitable for clinical application. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008

📜 SIMILAR VOLUMES

Effect of fibronectin on the adhesion of
Effect of fibronectin on the adhesion of an established cell line to a surface reactive biomaterial
✍ Seitz, T. L. ;Noonan, K. D. ;Hench, L. L. ;Noonan, N. E. 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 855 KB

## Abstract We have demonstrated that an established hamster cell line (NIL 8 M‐2) will adhere to the bioceramic bioglass. The rate at which the NIL 8 M‐2 cells assume a spread morphology on bioglass is density dependent and the morphology displayed by NIL 8 M‐2 cells attached to bioglass is much m

An evaluation of the cytotoxic effects o
An evaluation of the cytotoxic effects of orthodontic bonding adhesives upon a primary human oral gingival fibroblast culture and a permanent, human oral cancer-cell line
✍ Huang, Tsui-Hsien ;Tsai, Ching-Yi ;Chen, Shiow-Ling ;Kao, Chia-Tze 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 550 KB

## Abstract The purpose of this investigation was to determine the cytotoxic effects of three different kinds of orthodontic adhesive on a human primary gingival fibroblast culture (GF) and a human oral cancer‐cell line (OC2). The adhesives comprised a self‐cure bonding resin, a light‐cure bonding

Establishment and characterization of an
Establishment and characterization of an immortomouse-derived odontoblast-like cell line to evaluate the effect of insulin-like growth factors on odontoblast differentiation
✍ Javier Catón; Pablo Bringas Jr.; Margarita Zeichner-David 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 697 KB

## Abstract Insulin‐like growth factors (IGF‐I and IGF‐II) play important roles in regulating growth and differentiation of many different organs including teeth. The presence of these factors in the developing tooth has been demonstrated. In vitro studies using tooth explants grown in the presence