Cytotoxic t-cell precursors against non-immunogenic rat tumors: Limiting dilution analysis

✍ Scribed by M. Zöller

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: French
• Weight: 607 KB
• Volume: 37
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910370121

✦ Synopsis

After in vitro stimulation of lymphocytes with syngeneic tumor cells in the presence of interleukin 2 (IL-2). a cytotoxic T-cell response was observed against these spontaneously arising BDX tumors, which are non-immunogenic in the syngeneic host. The response was due to cytotoxic T-cells (CTL), which were specific for syngeneic tumor cells, i.e. neither syngeneic lymphoblasts, allogeneic lymphoblasts, allogeneic tumor cells, nor NK targets were lysed. The frequencies of anti-tumor lymph-node (LN) and spleen CTL precursors (CTL,,) against a panel of syngeneic tumors of different histology varied between 116,000 and I! 16,000. Further analysis of the reactivity pattern of anti-tumor CTL revealed some degree of cross-reactivity within the syngeneic system. By a limiting dilution (LD) split culture approach in combination with cold target (CT) inhibition, it could be shown that tumors carry a panel of tumor-associated antigens (TAAs). Some tumors express individually specific as well as cross-reactive TAAs, while others carry crossreactive TAAs only.