Quantitation of proliferative and cytotoxic precursor cells directed against human tumours: Limiting dilution analysis in peripheral blood and at the tumour site
✍ Scribed by Brent M. Vose
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- French
- Weight
- 741 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
Blood and tumour‐infiltrating lymphocytes (TIL) from 16 cancer patients have been examined under limiting dilution conditions to determine the frequency of cells responding in mixed tumour‐lymphocyte cultures (MLTC) to autologous tumour and Interleukin‐2 (IL‐2). Tumour‐derived lymphocytes showed a high spontaneous response to IL‐2 alone 1/1,900 in TIL; 1/6,000 in PBL suggesting the presence of “activated” T cells in situ. Proliferative frequencies were increased in MLTC in both blood (1/3,779) and TIL (1/1,084). Phenotypic analyses showed that total T‐cell contents of the responder populations were comparable but TIL were enriched for the OKT~8~^+^ subset with a corresponding reduction in OKT~4~^+^. TIL showed increased numbers of OKMI^+^ and Tac^+^ lymphocytes. The major cytotoxic precursor expanding under these conditions was reactive against autologous tumour. K562 (NK) were present at a lesser frequency ‐particularly in TIL The data show a concentration and activation of reactive lymphocytes at the tumour site and establish conditions for the clonal expansion of specifically cytotoxck T cells.