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Cytotoxic Properties of CD4+ T-Cell Clones Which Lyse HLA Class II Negative Autologous Non-Small-Cell Lung Cancer Cells

โœ Scribed by Claudia Semino; Guido Ferlazzo; Giovanni Battista Ratto; Giovanni Melioli


Book ID
112240171
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
79 KB
Volume
196
Category
Article
ISSN
0008-8749

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To generate non-small cell lung cancer (NSCLC)-reactive lymphocytes, we transfected an HLA-A2-expressing human NSCLC line (1355) with the cDNA encoding the lymphocyte co-stimulatory molecule CD80. Following selection in G418, 1355.7 demonstrated stable cell-surface expression of CD80. Allogeneic mix