Cytostatic peptide isolation from culture media of mouse peritoneal exudate cells
✍ Scribed by Ildikó Csuka; Ferenc Antoni; József Gróf
- Book ID
- 113133926
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 852 KB
- Volume
- 108
- Category
- Article
- ISSN
- 0008-8749
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A rapid method for the isolation of DNA from mouse cells grown in tissue culture is described. Cells are lysed in 0.24 M sodium phosphate, pH 6.8 buffer, containing 1% sodium dodecyl sulfate, 8 M urea, and 10m3 M ethylenediaminetetraacetic acid, and the crude lysate applied to a column of hydroxyapa