Cytostatic effect of different strains of Bacillus Calmette-Gu�rin on human bladder cancer cells in vitro alone and in combination with mitomycin C and Interferon-?

✍ Scribed by Rajala, P. ;Kaasinen, E. ;Rintala, E. ;Jauhiainen, K. ;Nurmi, M. ;Alfthan, O. ;L�hde, M.

• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 312 KB
• Volume: 20
• Category: Article
• ISSN: 0300-5623
• DOI: 10.1007/bf00299720

✦ Synopsis

The cytostatic activity of five Bacillus Calmette-Guérin (BCG) strains (Pasteur, Evans, Tice, RIVM and Connaught) on human transitional cell cancer T24 cells was examined. A striking effect was noted even in 2-day cultures, and the effect was more pronounced when the cells were incubated for 5 days with different BCG strains alone. The concentrations needed were about the same as those used in clinical practice (10(9) colony-forming units of Pasteur strain in 100 ml buffered saline solution). Combination with mitomycin C or interferon-alpha-2b potentiated the cytostatic effect. A slight difference in cytostatic activity between different BCG strains was found.