Cytoplasmic genetic diversity in bananas and plantains

✍ Scribed by N. Gawel; R. L. Jarret

• Book ID: 104617458
• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 506 KB
• Volume: 52
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00037852

✦ Synopsis

Concerns over yield declines in bananas and plantains due to the spread of Black Sigatoka disease in Musa have drawn attention to the collection of Musa germplasm and its use in conventional and biotechnological improvement programs . This report demonstrates the use of chloroplast DNA (cpDNA) restriction fragment length polymorphisms (RFLPs) for differentiating cytoplasms of various Musa clones . DNA was extracted from lyophilized leaf blade tissue and digested with either Eco R1, Hind III, Bam H1 or Pst I . Southern blots onto nylon membranes were probed with radioactively labeled heterologous orchid and lettuce cpDNA fragments . Among the 14 Musa clones examined, a single balbisiana and four acuminata-type cytoplasms were differentiated . The ability to distinguish between cytoplasms and to place plants within a cytoplasmic grouping demonstrates the usefulness of RFLP technology in evaluating diversity and determining the ancestry of Musa clones .