𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Cytolytic activity of circulating human monocytes on transformed and untransformed human fibroblasts

✍ Scribed by Alberto Mantovani; Aldo Tagliabue; Jack H. Dean; Thomas R. Jerrells; Ronald B. Herberman

Publisher
John Wiley and Sons
Year
1979
Tongue
French
Weight
402 KB
Volume
23
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Monocytes from the peripheral blood of 10 normal adult human donors were tested for their cytolytic activity on untransformed or SV40‐transformed, early passage, human fibroblasts. Two pairs of human fibroblast lines (2303 and SV40–2303, 2931 and SV40–2931) were used and lysis was assessed in terms of release of [^3^H]thymidine from prelabelled target cells over a 72‐h incubation period. High levels of cytolytic activity were consistently observed on transformed fibroblasts at attacker to target cell (A:T) ratios ranging from 5:1 to 40:1. Low, but significant, lysis of untransformed cells was observed only with two donors at the highest (40:1) A:T ratio tested. With all donors, the cytolytic activity on transformed lines was significantly greater than on untransformed fibroblasts. Thus natural human monocyte‐mediated cytotoxicity is preferentially expressed on transformed, as compared to untransformed, cells although sparing of the latter is not absolute.

πŸ“œ SIMILAR VOLUMES

Cytolytic and cytostatic activity on tum
Cytolytic and cytostatic activity on tumor cells of circulating human monocytes
✍ Alberto Mantovani; Thomas R. Jerrells; Jack H. Dean; Ronald B. Herberman πŸ“‚ Article πŸ“… 1979 πŸ› John Wiley and Sons 🌐 French βš– 980 KB

## Abstract Monocytes from the peripheral blood of normal adult human donors were found to have appreciable levels of cytotoxic activity against murine and human tumor‐cell lines. Adherent cells ( > 90% monocytes) were obtained from the peripheral blood of 29 normal healthy volunteers either by adh

Human keratinocyte growth-promoting acti
Human keratinocyte growth-promoting activity on the surface of fibroblasts
✍ Peter C. Yaeger; Charles D. Stiles; Barrett J. Rollins πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 944 KB

To proliferate in serum-containing medium, normal human keratinocytes must be co-cultured with fibroblast feeder cells. Conditioned medium from feeder cell cultures cannot substitute for the cells themselves. We tested the hypothesis that fibroblasts display a keratinocyte growth-promoting activity

Effects of several species of human leuk
Effects of several species of human leukocyte interferon on cytotoxic activity of NK cells and monocytes
✍ John R. Ortaldo; Alberto Mantovani; Donna Hobbs; Menachem Rubinstein; Sidney Pes πŸ“‚ Article πŸ“… 1983 πŸ› John Wiley and Sons 🌐 French βš– 497 KB πŸ‘ 1 views

## Abstract Ten species of purified human leukocyte interferon were tested for their ability to modulate the cytolytic activity of natural killer (NK) cells and the cytolytic and cytostatic activities of monocytes. The interferon species were tested at several antiviral tilers and examined for quan

Effect of aminonucleoside on transcripti
Effect of aminonucleoside on transcription, methylation, and maturation of ribosomal RNA in SV40-transformed human lung fibroblasts
✍ Allan R. Tunkel; George P. Studzinski πŸ“‚ Article πŸ“… 1981 πŸ› John Wiley and Sons 🌐 English βš– 890 KB

## Abstract The effects of puromycin aminonucleoside (AMS) have been studied in the nucleoli of SV40‐transformed human lung fibroblasts with attention to the relationships between transcription, methylation, and maturation of ribosomal RNA (rRNA). Inhibition of the transcription of pre‐rRNA became