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Cytogenetic and biological characterization of two new human plasma cell lines

โœ Scribed by L.W. Brox; A. Belch; E. Pollock; X.X. He; M. De Braekeleer; C.C. Lin


Book ID
119103634
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
630 KB
Volume
27
Category
Article
ISSN
0165-4608

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A stable line of IgC K producing human plasma cells was established from a myelomatous human bone marrow using conditioned media from a rapidly metabolizing lymphoblast line, RPMl 4098. Growth in RPMl 1640 (15% fetal calf serum) at 6% C02 promoted a 62-hour doubling time with a preferred cell concen