A human plasma cell line. Induction and characterization

✍ Scribed by C. H. Miller; A. Carbonell; R. Peng; T. Paglieroni; M. R. Mackenzie

• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 553 KB
• Volume: 49
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(19820515)49:10<2091::aid-cncr2820491021>3.0.co;2-9

✦ Synopsis

A stable line of IgC K producing human plasma cells was established from a myelomatous human bone marrow using conditioned media from a rapidly metabolizing lymphoblast line, RPMl 4098. Growth in RPMl 1640 (15% fetal calf serum) at 6% C02 promoted a 62-hour doubling time with a preferred cell concentration of 1 X 106/mL. Surface marker studies showed: no receptors for sheep erythrocytes, no surface immunoglobulins, variable number of cells bearing complement receptors and 83% bearing Fc receptors. Although transmission electron micrographs demonstrated a poorly developed endoplasmic reticulum, radioimmunoassay showed 23 ng IgG and 28.7 ng Kappa were produced by 1 X lo6 cells in 72 hours. Further, the cells are lipase, esterase and Epstein-Barr nuclear antigen negative. ASC banding showed a total chromosome number that varied from 46-49. Since the number of human plasma cell lines is limited, it is felt that this line will augment the immunobiological study of human myeloma.