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Cytochrome P450-dependent toxicity of dapsone in human erythrocytes

✍ Scribed by Shobana Ganesan; Rajnish Sahu; Larry A Walker; Babu L. Tekwani

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 246 KB
Volume: 30
Category: Article
ISSN: 0260-437X
DOI: 10.1002/jat.1493

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The most prominent adverse effects seen during treatment with dapsone, an antibacterial and antiprotozoal agent, are hemolysis and methemoglobinemia. An in vitro microsomal/cytochrome P~450~ (CYP)‐linked assay, which allows reactive metabolites generated in situ to react with the co‐incubated human erythrocytes, was employed to profile CYP isoforms responsible for hemotoxicity of dapsone. Dapsone caused a robust generation of methemoglobin in human erythrocytes in the presence of human/mouse liver microsomes, which indicates contribution of CYP‐mediated metabolism for hemotoxicity. The highest methemoglobin formation with dapsone was observed with CYP2C19, with minor contributions from CYP2B6, CYP2D6 and CYP3A4. Cimetidine and chloramphenicol completely abrogated methemoglobin generation by dapsone, thus confirming a predominant contribution of CYP2C19. The results provide useful insights into CYP‐dependent hemotoxicity of dapsone in human erythrocytes. Copyright © 2009 John Wiley & Sons, Ltd.

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