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Cytochrome c from Schizosaccharomyces pombe : 1. Purification, Spectral Properties, and Amino-Acid Composition

✍ Scribed by Maurice L. CLAISSE; Anne-Marie SIMON-BECAM


Book ID
115116685
Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
690 KB
Volume
86
Category
Article
ISSN
1432-1327

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Phosphatidylinositol-specific phospholipase C was purified in a 27 % yield from the culture medium of Bacillus cereus by a combination of ammonium sulfate precipitation and ion-exchange and hydrophobic interaction chromatography. The purified enzyme was free of other phospholipase C-type activities