Cytochalasin inhibition of isolated rat gastric parietal cell function

✍ Scribed by Gary C. Rosenfeld; Ellen McAllister; W. Joseph Thompson

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: English
• Weight: 391 KB
• Volume: 109
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041090107

✦ Synopsis

Abstract

Submicrogram concentrations (0.04‐‐0.29 μM) of the microfilament disrupting agents cytochalasins D, E, and B (CD, CE, CB) were shown to inhibit secretagogue‐stimulated ^14^C‐aminopyrine accumulation (AP) in isolated rat gastric mucosal parietal cells. The microtubule disrupting agent colchicine had little influence on AP accumulation. Histamine‐ and dibutyryl cyclic AMP (DbcAMP)‐stimulated AP accumulation was inhibited with an order of potency CD > CE ≈︁ CB. CB inhibition of these secretagogue actions was, however only approximately 65‐‐70% of the maximal stimulated response, whereas CD and CE caused 100% inhibition. On the other hand, carbamylcholine‐stimulated AP accumulation was inhibited 100% by all cytochalasins tested with an order of potency CD ≈︁ CE > CB. These data are discussed in relation to acid secretagogue‐induced morphological changes involving actin filament organization in parietal cells.