Cysteine enhances activity and stability of immobilized papain
β Scribed by Ahmad Abolpour Homaei; Reza H. Sajedi; Reyhaneh Sariri; Sara Seyfzadeh; Roberto Stevanato
- Book ID
- 106221854
- Publisher
- Springer
- Year
- 2009
- Tongue
- English
- Weight
- 293 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0939-4451
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## Abstract The effects of cysteine on dimethylarsinic acid (DMA)βinduced cytotoxicity and chromosomal aberration were studied using Chinese hamster V79 cells. The IC 50 of DMA, i.e. the concentration resulting in a 50% decrease in cell population of viable cells, was 130β Β΅gβ ml^β1^ (0.94 mM), where
## Abstract Papain, an endolytic cysteine protease (EC: 3.4.22.2), from __Carica papaya__ latex has been chemically modified using succinic anhydride. This reagent reacts with the amino group of the lysine residues in the enzyme, thereby changing its net charge from positive to negative. The result