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Cysteine Conjugate β-Lyase-Catalyzed Bioactivation of Bromine-Containing Cysteine S -Conjugates: Stoichiometry and Formation of 2,2-Difluoro-3-halothiiranes

✍ Scribed by Finkelstein, Martin B.; Dekant, Wolfgang; Anders, M. W.


Book ID
120539777
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
164 KB
Volume
9
Category
Article
ISSN
0893-228X

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An HPLC-fluorescence assay has been developed for the determination of the activity of rat renal cytosolic cysteine conjugate beta-lyase. The method is based on isocratic HPLC separation and fluorescence detection of pyruvic acid, derivatized with o-phenylenediamine (OPD), and is shown to be rapid,