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Cysteine Conjugate β-Lyase Activity of Rat Erythrocytes and Formation of β-Lyase-Derived Globin Monoadducts and Cross-Links after in Vitro Exposure of Erythrocytes to S-(1,2-Dichlorovinyl)- l -cysteine

✍ Scribed by Barshteyn, Nella; Elfarra, Adnan A.

Book ID: 127053464
Publisher: American Chemical Society
Year: 2009
Tongue: English
Weight: 633 KB
Volume: 22
Category: Article
ISSN: 0893-228X
DOI: 10.1021/tx9001429

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An HPLC-fluorescence assay has been developed for the determination of the activity of rat renal cytosolic cysteine conjugate beta-lyase. The method is based on isocratic HPLC separation and fluorescence detection of pyruvic acid, derivatized with o-phenylenediamine (OPD), and is shown to be rapid,