Cyclin D1 expression in astrocytomas is associated with cell proliferation activity and patient prognosis

✍ Scribed by Sallinen, Satu-Leena; Sallinen, Pauli K.; Kononen, Juha T.; Syrj�koski, Kirsi M.; Nupponen, Nina N.; Rantala, Immo S.; Hel�n, Pauli T.; Helin, Heikki J.; Haapasalo, Hannu K.

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 266 KB
• Volume: 188
• Category: Article
• ISSN: 0022-3417
• DOI: 10.1002/(sici)1096-9896(199907)188:3<289::aid-path351>3.0.co;2-x

✦ Synopsis

An important positive regulator of the cell cycle, cyclin D1, is often amplified and overexpressed in malignancies. Cyclin D1 aberrations were analysed in grade II-IV astrocytomas by fluorescence in situ hybridization (FISH), mRNA in situ hybridization and immunohistochemistry. Proliferation activity was determined by Ki-67 MIB-1 immunolabelling and mitotic counting. High cyclin D1 expression was observed in grade IV astrocytomas (grades II-III versus grade IV; mRNA expression: p<0•001; immunoexpression: p=0•013), and correlated with poor patient survival (p<0•001, n=46). Upregulated cyclin D1 expression was also closely associated with poor patient prognosis in grade II-III astrocytomas (p<0•001, n=30). Cyclin D1 gene was not found to be amplified (n=7). Cell proliferation activity was significantly increased in tumours exhibiting high cyclin D1 mRNA levels (Ki-67 MIB-1 : p<0•001; mitotic count: p<0•001) and high cyclin D1 protein expression (Ki-67 MIB-1 : p=0•002; mitotic count: p=0•012). These results indicate that increased production of cyclin D1 is closely associated with high cell proliferation activity and aggressive behaviour in diffusely infiltrating astrocytomas.