## Abstract Active transport of chloride in the salt secreting rectal gland of the dogfish __Squalus acanthias__ is markedly stimulated by the addition of theophylline (0.01 to 5 mM) or dibutyryl cyclic AMP (0.05 mM) during in vitro perfusion. The effect occurs promptly and may persist for two hour
Cyclic AMP and ouabain-binding sites in the rectal gland of the dogfishScyliorhinus canicula
✍ Scribed by Shuttleworth, T. J. ;Thompson, J. L.
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 256 KB
- Volume
- 206
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
A technique for measuring the binding of ^3^H ouabain to tissue from the elasmobranch rectal gland is described. Concentrations of dibutyryl cAMP and theophylline that have previously been shown to stimulate secretion from the perfused gland produce a large increase in the degree of ouabain binding and the implications of this in the possible control of secretion rate are discussed.
📜 SIMILAR VOLUMES
Vasoactive intestinal peptide (VIP) receptors and p-adrenergic receptors were investigated in rat Harderian gland membranes using '251-VIP and 125I-cyanopindolol(125I-CYP), respectively, as ligands. The receptor bindings were rapid, reversible, saturable, specific, and dependent on time, temperature
## Abstract cAMP is a well‐known regulator of exocytosis, and cAMP‐GEFII (Epac2) is involved in the potentiation of cAMP‐dependent, PKA‐independent regulated exocytosis in secretory cells. However, the mechanisms of its action are not fully understood. In the course of our study of Epac2 knockout m
## Abstract An intraperitoneal injection of the β‐adrenergic agonist dl‐isoproterenol hydrochloride (100 mg/Kg body weight) into a rat caused an early, very large (400‐fold) cyclic AMP surge (peaking at 10 minutes) in the parotid gland which was followed by a second, much smaller (two‐fold) surge 1