Rates of synthesis of cyclic 3',5'-adenosine monophosphate (cAMP) were measured in cultures of Escherichia coli aerating without a carbon source. This technique provides a representative measure of adenylate cyclase activity in the absence of inhibition caused by transport of the carbon source. Aden
Cyclic adenosine-3′,5′-monophosphate in cellular immunity
✍ Scribed by A. A. Hakim
- Publisher
- Springer
- Year
- 1974
- Tongue
- English
- Weight
- 286 KB
- Volume
- 61
- Category
- Article
- ISSN
- 0028-1042
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A column chromatogrnphic sy;itrm has been developed for isolation and purification of cAMP and cGMP simultnneously from small tissue samples. In this system, a column of neutral aluminum oxide equilibrated in Tris-HCl buffer, pH 7.5 was used to purify cyclic nucleotides from olhe~ nucleotides in non