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Current Protocols in Pharmacology || Cytochrome P450 Inhibition Assays Using Traditional and Fluorescent Substrates

โœ Scribed by Enna, S.J.; Williams, Michael; Barret, John F.; Ferkany, John W.; Kenakin, Terry; Porsolt, Roger D.


Book ID
125942493
Publisher
Wiley (John Wiley & Sons)
Year
2007
Tongue
English
Weight
246 KB
Volume
39
Category
Article
ISBN-13
9780471141754
ISSN
1934-8282

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A fast and robust LC/MS-based cytochrome P450 (CYP) inhibition assay, using human liver microsomes, has been fully developed and validated for the major human liver CYPs. Probe substrates were phenacetin, diclofenac, S-mephenytoin, and dextromethorphan for CYP1A2, CYP2C9, CYP2C19, and CYP2D6, respec