The ionization of nucleosides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was systematically investigated using adenine (A), thymine (T), guanine (G) and cytosine (C) with several common matrices. Experimental results of the protonation and deprotonation of the ba
Current Protocols in Nucleic Acid Chemistry || Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of Oligonucleotides
โ Scribed by Beaucage, Serge L.; Bergstrom, Donald E.; Glick, Gary D.; Jones, Roger A.
- Book ID
- 120252670
- Publisher
- John Wiley & Sons, Inc.
- Year
- 2008
- Weight
- 200 KB
- Volume
- 10.1002/0471142700
- Category
- Article
- ISBN-13
- 9780471142706
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Oligonucleotide sequencing by fragmentation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry has been demonstrated. With this sequencing method, the preparation of oligonucleotide ladder products and gel electrophoresis are not required, thus the sequencing
This study presents matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) as a powerful tool to analyze and characterize oligonucleotides covalently linked to a solid support during their synthesis. The analysis of the fragment ions generated either in negative o