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Current Protocols in Neuroscience || Selection of Transfected Mammalian Cells

โœ Scribed by Crawley, Jacqueline N.; Gerfen, Charles R.; Rogawski, Michael A.; Sibley, David R.; Skolnick, Phil; Wray, Susan

Book ID
118259237
Publisher
Wiley (John Wiley & Sons)
Year
1997
Tongue
English
Weight
246 KB
Volume
00
Category
Article
ISBN-13
9780471142300
ISSN
1934-8584

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Rescue of transfected genes from mammali
Rescue of transfected genes from mammalian cells by functional selection in Escherichia coli
โœ Strauss, Michael ;Klessling, Udo ;Kachler, Ruth ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Springer ๐ŸŒ English โš– 542 KB

We have established procedures for reisolating a transfected gene from mammalian cells by selection in Escherichia coli for the function of the gene product using the Herpes simplex virus thymidine kinase gene as a model. Rescue of the gene is accomplished by three different methods. The tk gene is