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Current Protocols in Cell Biology || Transfection by Electroporation

✍ Scribed by Bonifacino, Juan S.; Dasso, Mary; Harford, Joe B.; Lippincott-Schwartz, Jennifer; Yamada, Kenneth M.

Book ID
118253514
Publisher
John Wiley & Sons, Inc.
Year
2011
Weight
96 KB
Category
Article
ISBN-13
9780471143031

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## Abstract This unit presents two methods of calcium phosphate‐based eukaryotic cell transfection that can be used for both transient and stable transfections. In these protocols, plasmid DNA is introduced to monolayer cell cultures via a precipitate that adheres to the cell surface. A HEPES‐buffe