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Current Protocols in Molecular Biology || Calcium Phosphate Transfection

✍ Scribed by Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidman, J.G.; Smith, John A.; Struhl, Kevin

Book ID
118275842
Publisher
Wiley (John Wiley & Sons)
Year
2003
Tongue
English
Weight
147 KB
Volume
63
Category
Article
ISBN-13
9780471142720
ISSN
1934-3639

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Current Protocols in Molecular Biology |
Current Protocols in Molecular Biology || Calcium Phosphate Transfection
✍ Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidm πŸ“‚ Article πŸ“… 2003 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 147 KB

## Abstract This unit presents two methods of calcium phosphate‐based eukaryotic cell transfection that can be used for both transient and stable transfections. In these protocols, plasmid DNA is introduced to monolayer cell cultures via a precipitate that adheres to the cell surface. A HEPES‐buffe