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Current Protocols in Molecular Biology || Analysis of Oligosaccharide Negative Charge by Anion-Exchange Chromatography

✍ Scribed by Ausubel, Frederick M.; Brent, Roger; Kingston, Robert E.; Moore, David D.; Seidman, J.G.; Smith, John A.; Struhl, Kevin


Book ID
118277305
Publisher
Wiley (John Wiley & Sons)
Year
1995
Tongue
English
Weight
110 KB
Volume
32
Category
Article
ISBN-13
9780471142720
ISSN
1934-3639

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The on-line coupling of high-performance anion-exchange chromatography (HPAEC) with ion spray mass spectrometry, for the analysis of neutral and acidic oligosaccharides, is described. On-line desalting is performed by a cation-exchange membrane system which replaces sodium for hydronium ions from th