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Current Protocols in Cytometry || Intensity Calibration and Shading Correction for Fluorescence Microscopes

โœ Scribed by Robinson, J. Paul; Darzynkiewicz, Zbigniew; Dobrucki, Jurek; Hyun, William C.; Nolan, John P.; Orfao, Alberto; Rabinovitch, Peter S.

Book ID
121756525
Publisher
Wiley (John Wiley & Sons)
Year
2006
Tongue
English
Weight
139 KB
Volume
37
Category
Article
ISBN-13
9780471142959
ISSN
1934-9297

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A standard for calibration and shading c
A standard for calibration and shading correction of a fluorescence microscope
โœ Michael A. Model; Janis K. Burkhardt ๐Ÿ“‚ Article ๐Ÿ“… 2001 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 142 KB

Background: Numerous applications of fluorescence microscopy require quantitation of signal intensity in reproducible units. Two problems must be overcome to achieve this goal. First, due to various instrumental factors, the same sample imaged on two microscopes or even on the same microscope at dif